Journal: Cell Death & Disease

Article Title: Matrix stiffness regulates glucose-6-phosphate dehydrogenase expression to mediate sorafenib resistance in hepatocellular carcinoma through the ITGB1-PI3K/AKT pathway

doi: 10.1038/s41419-025-07842-3

Figure Lengend Snippet: A Venn diagram illustrating the overlap between genes related to ECM stiffness and those associated with sorafenib resistance. B Analysis of the top 20 KEGG pathways for genes related to ECM stiffness and sorafenib resistance. C Cross-validation plot for penalty terms. The cross-validation was used to select the optimum value of the regularization parameter ( λ ) in the LASSO regression model. D LASSO coefficient profiles of genes associated with ECM stiffness and sorafenib resistance. A coefficient profile plot was produced against the log( λ ) sequence. A vertical line was drawn at the selected optimal parameter ( λ ), which resulted in 12 nonzero coefficients. E A forest plot of 12 genes associated with ECM stiffness and sorafenib resistance. F Coefficients of these 12 genes. G Real-time qPCR analysis of gene expression related to ECM stiffness under soft (0.5 kPa) or stiff (12 kPa) matrix conditions. H Western blotting analysis of G6PD and AKR1B20 expression under soft (0.5 kPa) or stiff (12 kPa) matrix conditions. β-actin was used as a loading control and quantification data were shown on the right panel. I Representative images of H&E, Collagen I, α-SMA, Masson’s trichrome, Picrosirius red, AKR1B10, and G6PD staining in HCC tissues from patients who exhibited low stiffness and responsiveness to sorafenib and those with high stiffness and resistance to sorafenib. Scale bar, 200 and 500 μm as indicated. J Quantification of collagen I, α-SMA, Masson’s trichrome, and Picrosirius red area between 10 sorafenib-resistant samples with high ECM and 10 sorafenib-sensitive samples. K Association between AKR1B10 and G6PD expression levels and the degree of ECM stiffness in HCC tissue. ns not significance; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: The primary antibodies used were as follows: G6PD (Proteintech, 25413–1-AP, 1:500), AKR1B10 (Proteintech, 18252–1-AP, 1:2000), ITGB1 (Proteintech, 12594–1-AP, 1:1000), collagen I (Proteintech, 10176–2-AP, 1:2500), α-SMA (Proteintech, 66009–1-Ig, 1:500), and p-AKT (Ser473, Proteintech, 66444–1-Ig, 1:2000).

Techniques: Biomarker Discovery, Produced, Sequencing, Gene Expression, Western Blot, Expressing, Control, Staining

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Primer sequences for real-time PCR amplification.

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Real-time Polymerase Chain Reaction, Amplification

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid and small peptide transporters in the jejunum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid and small peptide transporters in the ileum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid and small peptide transporters in the jejunum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Representative immunoblots of ZnT1, ZnT4, ZnT5, ZnT7, ZnT9, ZIP3, ZIP5, B 0 AT1, LAT1, y+LAT2, rBAT and PepT1 in the jejunum of broilers at 28 (A) and 39 d (B) of age. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B 0 AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b 0 , + -type amino acid transporter; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6).

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Western Blot

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid and small peptide transporters in the ileum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Representative immunoblots of ZnT1, ZnT4, ZnT5, ZnT7, ZnT9, ZIP3, ZIP5, B 0 AT1, LAT1, y+LAT2, rBAT and PepT1 in the ileum of broilers at 28 (A) and 39 d (B) of age. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B 0 AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b 0 , + -type amino acid transporter; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6).

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Western Blot